Experimental study on inducing apoptosis and reversing drug resistance of gastric cancer cells SGC7901 by antisense Survivin RNA
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چکیده
منابع مشابه
Survivin antisense oligodeoxynucleotide inhibits growth of gastric cancer cells.
AIM To investigate the effect of transfected survivin antisense oligonucleotide (ASODN) on proliferation and apoptosis of gastric cancer cells. METHODS The authors designed ASODNs targeting different regions of survivin mRNA, including surviving ASODN1, ASODN2 and ASODN3. ASODNs were transfected into gastric cancer cell line SGC 7901, cell growth was detected by MTT assay. Cells exposed to th...
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AIM To explore the reversal effect of mifepristone on multidrug resistance (MDR) in drug-resistant human gastric cancer cell line SGC7901/VCR and its mechanisms. METHODS Expression of multidrug resistance-associated protein(MRP) was detected using reverse transcription-polymerase chain reaction(RT-PCR). Flow cytometry was used to assay the expression of P-glycoprotein(P-gp), Bcl-2, Bax, and t...
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Gastric carcinoma, also known as stomach carcinoma, is the fourth most common cancer in the world (Brenner et al., 2009). In China, gastric carcinoma is the second most common cancer (Zhao et al., 2010). It is reported that almost two thirds of the cases of this type of cancer occur in developing countries and 42% in China alone. Despite a major decline in incidence and mortality over the past ...
متن کاملAndrographolide Inhibits Proliferation and Metastasis of SGC7901 Gastric Cancer Cells
To explore the mechanisms by which andrographolide inhibits gastric cancer cell proliferation and metastasis, we employed the gastric cell line SGC7901 to investigate the anticancer effects of andrographolide. The cell survival ratio, cell migration and invasion, cell cycle, apoptosis, and matrix metalloproteinase activity were assessed. Moreover, western blotting and real-time PCR were used to...
متن کاملTGF-β1 promotes human gastric carcinoma SGC7901 cells invasion by inducing autophagy.
OBJECTIVE To investigate the role of TGF-b1 in autophagy and invasion ability in human gastric carcinoma cell line SGC7901. MATERIALS AND METHODS Cultured SGC7901 cells were treated with different concentrations of TGF-b1 for 24 h. The protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western blot. The effect of TGF-b1 on invasion ability of SGC7901 cells...
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ژورنال
عنوان ژورنال: World Chinese Journal of Digestology
سال: 2006
ISSN: 2219-2859
DOI: 10.11569/wcjd.v14.i12.1139